Analysis of Pasteurella Multocida Strains Isolated from Cattle with Haemorrhagic Septicaemia by the Use of Pfge
نویسنده
چکیده
Forty Pasteurella multocida subsp. multocida strains collected from cattle with haemorrhagic septicaemia belonging to serotype B:2, 5 and two reference strains of serotype B:2, were examined. The strains were isolated in the years 1958 to 1962 and 1985 to 1995. Analysis of the polimorfism of restricted DNA fragments by the use of the pulsed-field gel electrophoresis (PFGE) technique of contour-clamped homogeneous electric field electrophoresis were made. The phylogenetic relationship between the examined strains was calculated by the unweighted pair-group method using arithmetic averages After digestion of chromosomal DNA by using ApaI endonuclease, field isolates from HS cases were homogenous. The presence of an additional restrictive fragment in electrophoretic profile was found only in one strain.
منابع مشابه
Pathogenicity and immunogenicity of native and mutant strains of Pasteurella multocida, the causative agents of haemorrhagic septicaemia
Haemorrhagic septicaemia (HS) is a fatal systemic disease of cattle and buffaloes. Some control isachieved with administration of alum-precipitated or oil-adjuvanted killed whole-cell vaccines injectedsubcutaneously. These vaccines, however, provide only short-term immunity and for effective use, theyshould be administered annually. We constructed an aroA attenuated derivative of a Pasteurella ...
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